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hepes buffer  (Addgene inc)


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    Structured Review

    Addgene inc hepes buffer
    Hepes Buffer, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pca+g+intron+g/pCA-G-intron(Neo)-T(FLLFLNL)+(Plasmid+%2340020)/bio_rxiv__64898__2025__12__05__692577-334-48-8
    Average 93 stars, based on 1 article reviews
    hepes buffer - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: G-quadruplexes offer a conserved structural motif for NONO recruitment to NEAT1 architectural lncRNA
    Article Snippet: .. The pcDNA3.0 backbone was linearized with XbaI and BamHI and a GFP-coding sequence containing an intron (‘eG-FP’), from pCA-G-intron-G ( ) (gift from Liqun Luo, Addgene # 40027), and a 3′ EcoRI site was inserted using Gibson assembly to yield pCDNA3.0-eG-FP. .. A tRNA scaffolded SA ('tRSA') sequence was amplified from pcDNA3-tRSA ( ) (gift from Ian Macara, Addgene # 32200) and ligated into the EcoRI site of pCDNA3.0-eG-FP to produce pcDNA3.0-eG-FP-tRSA. (GGGGCC) 70 sequence flanked by EcoRI sites was cut from pCR8-70 ( ) and inserted into the EcoRI site of pcDNA3.0-eG-FP-tRSA to produce pCDNA3.0-eG-FP-tRSA-(GGGGCC) 70 .

    Gibson Assembly:

    Article Title: G-quadruplexes offer a conserved structural motif for NONO recruitment to NEAT1 architectural lncRNA
    Article Snippet: .. The pcDNA3.0 backbone was linearized with XbaI and BamHI and a GFP-coding sequence containing an intron (‘eG-FP’), from pCA-G-intron-G ( ) (gift from Liqun Luo, Addgene # 40027), and a 3′ EcoRI site was inserted using Gibson assembly to yield pCDNA3.0-eG-FP. .. A tRNA scaffolded SA ('tRSA') sequence was amplified from pcDNA3-tRSA ( ) (gift from Ian Macara, Addgene # 32200) and ligated into the EcoRI site of pCDNA3.0-eG-FP to produce pcDNA3.0-eG-FP-tRSA. (GGGGCC) 70 sequence flanked by EcoRI sites was cut from pCR8-70 ( ) and inserted into the EcoRI site of pcDNA3.0-eG-FP-tRSA to produce pCDNA3.0-eG-FP-tRSA-(GGGGCC) 70 .



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